Reviews

Maas S.L., Breakefield X.O. & Weaver A.M.

Extracellular vesicles: Unique intercellular delivery vehicles

Trends Cell Biol (2017) 27:172-188.

Matsuda A., Yan I.K., Foye C., Parasramka M. & Patel T.

MicroRNAs as paracrine signaling mediators in cancers and metabolic diseases

Best Pract Res Clin Endocrinol Metab (2016) 30:577-590.

Nazimek K., Bryniarski K. & Askenase P.W.

Functions of exosomes and microbial extracellular vesicles in allergy and contact and delayed-type hypersensitivity

Int Arch Allergy Immunol (2016) 171:1-26.

Shah R., Pico A.R. & Freedman J.E.

Translational epidemiology: Entering a Brave New World of Team Science

Circ Res (2016) 119:1060-1062.

Shah M.Y., Ferrajoli A., Sood A.K., Lopez-Berestein G. & Calin G.A.

microRNA therapeutics in cancer - An emerging concept

EBioMedicine (2016) 12:34-42.

Hatakeyama H., Wu S.Y., Mangala L.S., Lopez-Berestein G. & Sood A.K.

Assessment of in vivo siRNA delivery in cancer mouse models

Methods Mol Biol (2016) 1402:189-97.

Hall J., Prabhakar S., Balaj L., Lai C.P., Cerione R.A. & Breakefield X.O.

Delivery of therapeutic proteins via extracellular vesicles: Review and potential treatments for Parkinson's disease, glioma, and Schwannoma

Cell Mol Neurobiol (2016) 36:417-27.

Rennert R.C., Hochberg F.H. & Carter B.S.

ExRNA in biofluids as biomarkers for brain tumors

Cell Mol Neurobiol (2016) 36:353-60.

Parasramka M.A., Maji S., Matsuda A., Yan I.K. & Patel T.

Long non-coding RNAs as novel targets for therapy in hepatocellular carcinoma

Pharmacol Ther (2016) 161:67-78. Related Pathway

 

Mohankumar S. & Patel T.

Extracellular vesicle long noncoding RNA as potential biomarkers of liver cancer

Brief Funct Genomics (2016) 15:249-56.

Byron S.A., Van Keuren-Jensen K.R., Engelthaler D.M., Carpten J.D. & Craig D.W.

Translating RNA sequencing into clinical diagnostics: Opportunities and challenges

Nat Rev Genet (2016) 17:257-271. Related blog post

Rupaimoole R., Calin G.A., Lopez-Berestein G. & Sood A.K.

miRNA deregulation in cancer cells and the tumor microenvironment

Cancer Discov (2016) 6:235-46.

Hansen J.M., Coleman R.L. & Sood A.K.

Targeting the tumour microenvironment in ovarian cancer

Eur J Cancer (2016) 56:131-43.

Clancy L. & Freedman J.E.

Blood-Derived extracellular RNA and platelet pathobiology: Adding pieces to a complex circulating puzzle

Circ Res (2016) 118:374-6.

Guo P., Noji H., Yengo C.M., Zhao Z. & Grainge I.

Biological nanomotors with a revolution, linear, or rotation motion mechanism

Microbiol Mol Biol Rev (2016) 80:161-86.

Fabris L., Ceder Y., Chinnaiyan A.M., Jenster G.W., Sorensen K.D., Tomlins S., Visakorpi T. & Calin G.A.

The potential of microRNAs as prostate cancer biomarkers

Eur Urol AOP 2016-01-21.

Ainsztein A.M., Brooks P.J., Dugan V.G., Ganguly A., Guo M., Howcroft T.K., Kelley C.A., Kuo L.S., Labosky P.A., Lenzi R., McKie G.A., Mohla S., Procaccini D., Reilly M., Satterlee J.S., Srinivas P.R., Church E.S., Sutherland M., Tagle D.A., Tucker J.M. & Venkatachalam S.

The NIH Extracellular RNA Communication Consortium

J Extracell Vesicles (2015) 4:27493.

Mitamura T., Gourley C. & Sood A.K.

Prediction of anti-angiogenesis escape

Gynecol Oncol (2016) 141:80-85.

Nouraee N., Mowla S.J. & Calin G.A.

Tracking miRNAs' footprints in tumor-microenvironment interactions: Insights and implications for targeted cancer therapy

Genes Chromosomes Cancer (2015) 54:335-52.

Pua H.H. & Ansel K.M.

MicroRNA regulation of allergic inflammation and asthma

Curr Opin Immunol (2015) 36:101-8.

Simpson L.J. & Ansel K.M.

MicroRNA regulation of lymphocyte tolerance and autoimmunity

J Clin Invest (2015) 125:2242-9.

Saugstad J.A.

Non-Coding RNAs in stroke and neuroprotection

Front Neurol (2015) 6:50.

Tewari M.

A functional extracellular transcriptome in animals? Implications for biology, disease and medicine

Genome Biol (2015) 16:47.

Kawikova I. & Askenase P.W.

Diagnostic and therapeutic potentials of exosomes in CNS diseases

Brain Res (2015) 1617:63-71.

Godlewski J., Krichevsky A.M., Johnson M.D., Chiocca E.A. & Bronisz A.

Belonging to a network--microRNAs, extracellular vesicles, and the glioblastoma microenvironment

Neuro Oncol (2015) 17:652-62.

Xiao J., Sluijter J.P., Das S., Yang Y. & Shen Z.

A snapshot of genetic and epigenetic basis of arrhythmia and heart failure

Front Genet (2015) 6:74.

Srivastava A., Filant J., Moxley K.M., Sood A., McMeekin S. & Ramesh R.

Exosomes: a role for naturally occurring nanovesicles in cancer growth, diagnosis and treatment

Curr Gene Ther (2015) 15:182-92.

Danielson K.M. & Das S.

Extracellular vesicles in heart disease: Excitement for the future?

Exosomes Microvesicles (2014) 2:1. Related Pathway

 

Rajendran L., Bali J., Barr M.M., Court F.A., Kramer-Albers E.M., Picou F., Raposo G., van d.e.r., van N.i.e.l., Wang J. & Breakefield X.O.

Emerging roles of extracellular vesicles in the nervous system

J Neurosci (2014) 34:15482-9.

Gangoda L., Boukouris S., Liem M., Kalra H. & Mathivanan, S.

Extracellular vesicles including exosomes are mediators of signal transduction: Are they protective or pathogenic?

Proteomics. (2015) 15:260-271. Related Pathway

 

Quesenberry P.J., Goldberg L.R. & Dooner M.S.

Concise reviews: A stem cell apostasy: a tale of four H words

Stem Cells (2015) 33:15-20.

Saleem S.N. & Abdel-Mageed A.B.

Tumor-derived exosomes in oncogenic reprogramming and cancer progression

Cell Mol Life Sci (2015) 72:1-10.

Koupenova M. & Freedman J.E.

Platelets: the unsung hero of the immune response

J Thromb Haemost (2015) 13:268-70.

Gharpure K.M., Wu S.Y., Li C., Lopez-Berestein G. & Sood A.K.

Nanotechnology: Future of oncotherapy

Clin Cancer Res (2015) 21:3121-30.

Maguire C.A., Ramirez S.H., Merkel S.F., Sena-Esteves M. & Breakefield X.O.

Gene therapy for the nervous system: challenges and new strategies

Neurotherapeutics (2014) 11:817-39.

Berindan-Neagoe I., Monroig P.d.e.l., Pasculli B. & Calin G.A.

MicroRNAome genome: a treasure for cancer diagnosis and therapy

CA Cancer J Clin (2014) 64:311-36.

Berindan-Neagoe I. & Calin G.A.

Molecular pathways: microRNAs, cancer cells, and microenvironment

Clin Cancer Res (2014) 20:6247-53.

Quesenberry P.J., Goldberg L., Aliotta J. & Dooner M.

Marrow hematopoietic stem cells revisited: They exist in a continuum and are not defined by standard purification approaches; Then there are the microvesicles

Front Oncol (2014) 4:56.

Quesenberry P.J., Goldberg L.R., Aliotta J.M., Dooner M.S., Pereira M.G., Wen S. & Camussi G.

Cellular phenotype and extracellular vesicles: basic and clinical considerations

Stem Cells Dev (2014) 23:1429-36.

Davis A.N., Afshar-Kharghan V. & Sood A.K.

Platelet effects on ovarian cancer

Semin Oncol (2014) 41:378-84.

Thomas M.F., L'Etoile N.D. & Ansel K.M.

Eri1: a conserved enzyme at the crossroads of multiple RNA-processing pathways

Trends Genet (2014) 30:298-307. Related Pathway

 

Yakob M., Fuentes L., Wang M.B., Abemayor E. & Wong D.T.

Salivary biomarkers for detection of oral squamous cell carcinoma - current state and recent advances

Curr Oral Health Rep (2014) 1:133-141.

Redzic J.S., Balaj L., van d.e.r. & Breakefield X.O.

Extracellular RNA mediates and marks cancer progression

Semin Cancer Biol (2014) 28:14-23.

Freedman J.E.

Inherited dysfunctional nitric oxide signaling and the pathobiology of atherothrombotic disease

Circ Res (2014) 114:1372-3.

Patel T.

Extracellular vesicle noncoding RNA: New players in the diagnosis and pathogenesis of cholangiocarcinoma

Hepatology (2014) 60:782-4.

Hochberg F.H., Atai N.A., Gonda D., Hughes M.S., Mawejje B., Balaj L. & Carter R.S.

Glioma diagnostics and biomarkers: an ongoing challenge in the field of medicine and science

Expert Rev Mol Diagn (2014) 14:439-52.

Vader P., Breakefield X.O. & Wood M.J.

Extracellular vesicles: emerging targets for cancer therapy

Trends Mol Med (2014) 20:385-393.

Dalton H.J., Armaiz-Pena G.N., Gonzalez-Villasana V., Lopez-Berestein G., Bar-Eli M. & Sood A.K.

Monocyte subpopulations in angiogenesis

Cancer Res (2014) 74:1287-93.

Pusic A.D., Pusic K.M. & Kraig R.P.

What are exosomes and how can they be used in multiple sclerosis therapy?

Expert Rev Neurother (2014) 14:353-5.

Morey R. & Laurent L.C.

Getting off the ground state: X chromosome inactivation knocks down barriers to differentiation

Cell Stem Cell (2014) 14:131-2.

Takahashi K., Yan I., Haga H. & Patel T.

Long noncoding RNA in liver diseases

Hepatology (2014) 60:744-53. Related Pathway

 

Clancy L. & Freedman J.E.

New paradigms in thrombosis: novel mediators and biomarkers platelet RNA transfer

J Thromb Thrombolysis (2014) 37:12-6.

Ozpolat B., Sood A.K. & Lopez-Berestein G.

Liposomal siRNA nanocarriers for cancer therapy

Adv. Drug Deliv. Rev. (2014) 66:110-116. Related Pathway

 

Giza D.E., Vasilescu C. & Calin G.A.

Key principles of miRNA involvement in human diseases

Discoveries (Craiova) (2014) 2:e34.

Morey R. & Laurent L.C.

Getting off the ground state: X chromosome inactivation knocks down barriers to differentiation

Cell Stem Cell (2014) 14:131-2.

Patel T. & Harnois D.

Assessment of response to therapy in hepatocellular carcinoma

Ann Med (2014) 46:130-7.

Camussi G. & Quesenberry P.J.

Perspectives on the potential therapeutic uses of vesicles

Exosomes Microvesicles (2013) 1:6.

Aslan B., Ozpolat B., Sood A.K. & Lopez-Berestein G.

Nanotechnology in cancer therapy

J Drug Target (2013) 21:904-13.

Saugstad J.A.

MicroRNAs as effectors of brain function

Stroke (2013) 44:S17-9.

Lai C.P. & Breakefield X.O.

Role of exosomes/microvesicles in the nervous system and use in emerging therapies

Front Physiol (2012) 3:228.

Mechanisms / Biogenesis

Harjes U., et al.

Antiangiogenic and tumour inhibitory effects of downregulating tumour endothelial FABP4

Oncogene (2017) 36:912-921.

Teng Y., et al.

MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression

Nat Commun (2017) 8:14448.

Seviour E.G., et al.

Targeting KRas-dependent tumour growth, circulating tumour cells and metastasis in vivo by clinically significant miR-193a-3p

Oncogene (2017) 36:1339-1350.

Van Roosbroeck K., et al.

Combining anti-miR-155 with chemotherapy for the treatment of lung cancers

Clin Cancer Res AOP 2016-11-30.

Dou Y., et al.

Circular RNAs are down-regulated in KRAS mutant colon cancer cells and can be transferred to exosomes

Sci Rep (2016) 6:37982.

Haraszti R.A., et al.

High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources

J Extracell Vesicles (2016) 5:32570.

Hatakeyama H., et al.

Role of CTGF in sensitivity to hyperthermia in ovarian and uterine cancers

Cell Rep (2016) 17:1621-1631.

Ohtsuka M., et al.

H19 noncoding RNA, an independent prognostic factor, regulates essential Rb-E2F and CDK8-beta-catenin signaling in colorectal cancer

EBioMedicine (2016) 13:113-124.

Tao L., et al.

Crucial role of miR-433 in regulating cardiac fibrosis

Theranostics (2016) 6:2068-2083.

Chaluvally-Raghavan P., et al.

Direct upregulation of STAT3 by microRNA-551b-3p deregulates growth and metastasis of ovarian cancer

Cell Rep (2016) 15:1493-504.

McKenzie A.J., Hoshino D., Hong N.H., Cha D.J., Franklin J.L., Coffey R.J., Patton J.G. & Weaver A.M.

KRAS-MEK signaling controls Ago2 sorting into exosomes

Cell Rep (2016) 15:978-87.

Clarke D., Sethi A., Li S., Kumar S., Chang R.W., Chen J. & Gerstein M.

Identifying allosteric hotspots with dynamics: Application to inter- and intra-species conservation

Structure (2016) 24:826-37.

Parasramka M.A., Maji S., Matsuda A., Yan I.K. & Patel T.

Long non-coding RNAs as novel targets for therapy in hepatocellular carcinoma

Pharmacol Ther (2016) 161:67-78. Related Pathway

 

Pucci F., Garris C., Lai C.P., Newton A., Pfirschke C., Engblom C., Alvarez D., Sprachman M., Evavold C., Magnuson A., von Andrian U.H., Glatz K., Breakefield X.O., Mempel T.R., Weissleder R. & Pittet M.J.

SCS macrophages suppress melanoma by restricting tumor-derived vesicle-B cell interactions

Science (2016) 352:242-6.

Kalani M.Y., Siniard A.L., Corneveaux J.J., Bruhns R., Richholt R., Forseth J., Zabramski J.M., Nakaji P., Spetzler R.F. & Huentelman M.J.

Rare variants in cardiomyopathy genes associated with stress-induced cardiomyopathy

Neurosurgery (2016) 78:835-43.

Pan Y., et al.

miR-509-3p is clinically significant and strongly attenuates cellular migration and multi-cellular spheroids in ovarian cancer

Oncotarget (2016) 7:25930-48.

Teng Y., et al.

Grapefruit-derived nanovectors deliver miR-18a for treatment of liver metastasis of colon cancer by induction of M1 macrophages

Oncotarget (2016) 7:25683-97.

Pua H.H., et al.

MicroRNAs 24 and 27 suppress allergic inflammation and target a network of regulators of T Helper 2 cell-associated cytokine production

Immunity (2016) 44:821-32.

Pusic K.M., Pusic A.D. & Kraig R.P.

Environmental Enrichment stimulates immune cell secretion of exosomes that promote CNS myelination and may regulate inflammation

Cell Mol Neurobiol (2016) 36:313-325.

Zhang X., Abels E.R., Redzic J.S., Margulis J., Finkbeiner S. & Breakefield X.O.

Potential transfer of polyglutamine and CAG-repeat RNA in extracellular vesicles in Huntington's disease: Background and evaluation in cell culture

Cell Mol Neurobiol (2016) 36:459-470.

Iavello A., Frech V.S., Gai C., Deregibus M.C., Quesenberry P.J. & Camussi G.

Role of Alix in miRNA packaging during extracellular vesicle biogenesis

Int J Mol Med (2016) 37:958-966.

Rupaimoole R., Calin G.A., Lopez-Berestein G. & Sood A.K.

miRNA deregulation in cancer cells and the tumor microenvironment

Cancer Discov (2016) 6:235-246.

Guo P., Noji H., Yengo C.M., Zhao Z. & Grainge I.

Biological nanomotors with a revolution, linear, or rotation motion mechanism

Microbiol Mol Biol Rev (2016) 80:161-186.

Danielson K.M., Estanislau J., Tigges J., Toxavidis V., Camacho V., Felton E.J., Khoory J., Kreimer S., Ivanov A.R., Mantel P.Y., Jones J., Akuthota P., Das S. & Ghiran I.

Diurnal variations of circulating extracellular vesicles measured by nano flow cytometry

PLoS ONE (2016) 11:e0144678.

Ling H., Pickard K., Ivan C., Isella C., Ikuo M., Mitter R., Spizzo R., Bullock M.D., Braicu C., Pileczki V., Vincent K., Pichler M., Stiegelbauer V., Hoefler G., Almeida M.I., Hsiao A., Zhang X., Primrose J.N., Packham G.K., Liu K., Bojja K., Gafa R., Xiao L., Rossi S., Song J.H., Vannini I., Fanini F., Kopetz S., Zweidler-McKay P., Wang X., Ionescu C., Irimie A., Fabbri M., Lanza G., Hamilton S.R., Berindan-Neagoe I., Medico E., Mirnezami A.H., Calin G.A. & Nicoloso M.S.

The clinical and biological significance of MIR-224 expression in colorectal cancer metastasis

Gut (2016) 65:977-989.

Rupaimoole R., et al.

Hypoxia-upregulated microRNA-630 targets Dicer, leading to increased tumor progression

Oncogene AOP 2016-01-04. Related blog post

Wang J.Q., et al.

Extracellular vesicle-mediated reversal of Paclitaxel resistance in prostate cancer

Crit Rev Oncog (2015) 20:407-17.

Rupaimoole R., et al.

Long noncoding RNA ceruloplasmin promotes cancer growth by altering glycolysis

Cell Rep (2015) 13:2395-402.

Shields B.B., Pecot C.V., Gao H., McMillan E., Potts M., Nagel C., Purinton S., Wang Y., Ivan C., Kim H.S., Borkowski R.J., Khan S., Rodriguez-Aguayo C., Lopez-Berestein G., Lea J., Gazdar A., Baggerly K.A., Sood A.K. & White M.A.

A genome-scale screen reveals context-dependent ovarian cancer sensitivity to miRNA overexpression

Mol Syst Biol (2015) 11:842. Related Pathways: 1, 2

 

Pusic A.D. & Kraig R.P.

Phasic treatment with Interferon Gamma stimulates release of exosomes that protect against spreading depression

J Interferon Cytokine Res (2015) 35:795-807.

Patton J.G., Franklin J.L., Weaver A.M., Vickers K., Zhang B., Coffey R.J., Ansel K.M., Blelloch R., Goga A., Huang B., L'Etoille N., Raffai R.L., Lai C.P., Krichevsky A.M., Mateescu B., Greiner V.J., Hunter C., Voinnet O. & McManus M.T.

Biogenesis, delivery, and function of extracellular RNA

J Extracell Vesicles (2015) 4:27494.

Cha D.J., Franklin J.L., Dou Y., Liu Q., Higginbotham J.N., Demory B.e.c.k.l.e.r., Weaver A.M., Vickers K., Prasad N., Levy S., Zhang B., Coffey R.J. & Patton J.G.

KRAS-dependent sorting of miRNA to exosomes

Elife (2015) 4:e07197.

Sung B.H., Ketova T., Hoshino D., Zijlstra A. & Weaver A.M.

Directional cell movement through tissues is controlled by exosome secretion

Nat Commun (2015) 6:7164.

Bryniarski K., Ptak W., Martin E., Nazimek K., Szczepanik M., Sanak M. & Askenase P.W.

Free extracellular miRNA functionally targets cells by transfecting exosomes from their companion cells

PLoS ONE (2015) 10:e0122991.

Agarwal K., Saji M., Lazaroff S.M., Palmer A.F., Ringel M.D. & Paulaitis M.E.

Analysis of exosome release as a cellular response to MAPK pathway inhibition

Langmuir (2015) 31:5440-8.

Miyake T., Pradeep S., Wu S.Y., Rupaimoole R.., Zand B., Wen Y., Gharpure K.M., Nagaraja A.S., Hu W., Cho M.S., Dalton H.J., Previs R.A., Taylor M.L., Hisamatsu T., Kang Y., Liu T., Shacham S., McCauley D., Hawke D.H., Wiktorowicz J.E., Coleman R.L., & Sood A.K.

XPO1/CRM1 inhibition causes antitumor effects by mitochondrial accumulation of eIF5A

Clin Cancer Res (2015) 21:3286-3297. Related Pathway

 

Li K., Ching D., Luk F.S. & Raffai R.L.

Apolipoprotein E enhances microRNA-146a in monocytes and macrophages to suppress nuclear factor-kappaB-driven inflammation and atherosclerosis

Circ Res (2015) 117:e1-e11. Related Pathway

 

Wong H.K., Fatimy R.E., Onodera C., Wei Z., Yi M., Mohan A., Gowrisankaran S., Karmali P., Marcusson E., Wakimoto H., Stephens R., Uhlmann E.J., Song J.S., Tannous B. & Krichevsky A.M.

The Cancer Genome Atlas analysis predicts microRNA for targeting cancer growth and vascularization in glioblastoma

Mol Ther (2015) 23:1234-47. Related Pathway

 

Huan T., Rong J., Liu C., Zhang X., Tanriverdi K., Joehanes R., Chen B.H., Murabito J.M., Yao C., Courchesne P., Munson P.J., O'Donnell C.J., Cox N., Johnson A.D., Larson M.G., Levy D. & Freedman J.E.

Genome-wide identification of microRNA expression quantitative trait loci

Nat Commun (2015) 6:6601.

Gonzalez-Villasana V., Fuentes-Mattei E., Ivan C., Dalton H.J., Rodriguez-Aguayo C., Fernandez-de Thomas R.J., Aslan B., Del C Monroig P., Velazquez-Torres G., Previs R.A., Pradeep S., Kahraman N., Wang H., Kanlikilicer P., Ozpolat B., Calin G., Sood A.K., & Lopez-Berestein G.

Rac1/Pak1/p38/MMP-2 axis regulates angiogenesis in ovarian cancer

Clin Cancer Res (2015) 21:2127-2137. Related Pathway

 

Kanada M., Bachmann M.H., Hardy J.W., Frimannson D.O., Bronsart L., Wang A., Sylvester M.D., Schmidt T.L., Kaspar R.L., Butte M.J., Matin A.C. & Contag C.H.

Differential fates of biomolecules delivered to target cells via extracellular vesicles

Proc Natl Acad Sci U S A (2015) 112:E1433-42.

Deng Z., Mu J., Tseng M., Wattenberg B., Zhuang X., Egilmez N.K., Wang Q., Zhang L., Norris J., Guo H., Yan J., Haribabu B., Miller D. & Zhang H.G.

Enterobacteria-secreted particles induce production of exosome-like S1P-containing particles by intestinal epithelium to drive Th17-mediated tumorigenesis

Nat Commun (2015) 6:6956.

Collino F., Bruno S., Incarnato D., Dettori D., Neri F., Provero P., Pomatto M., Oliviero S., Tetta C., Quesenberry P.J. & Camussi G.

AKI recovery induced by mesenchymal stromal cell-derived extracellular vesicles carrying microRNAs

J Am Soc Nephrol (2015) 26:2349-2360.

Liu X., Xiao J., Zhu H., Wei X., Platt C., Damilano F., Xiao C., Bezzerides V., Bostrom P., Che L., Zhang C., Spiegelman B.M. & Rosenzweig A.

miR-222 is necessary for exercise-induced cardiac growth and protects against pathological cardiac remodeling

Cell Metab (2015) 21:584-95. Related Pathway

 

Cox J.E., McClure L.V., Goga A. & Sullivan C.S.

Pan-viral-microRNA screening identifies interferon inhibition as a common function of diverse viruses

Proc Natl Acad Sci U S A (2015) 112:1856-61.

Ren A., Xue Y., Peselis A., Serganov A., Al-Hashimi H.M. & Patel D.J.

Structural and dynamic basis for low-affinity, high-selectivity binding of L-glutamine by the glutamine riboswitch

Cell Rep (2015) 13:1800-13.

Shields B.B., Pecot C.V., Gao H., McMillan E., Potts M., Nagel C., Purinton S., Wang Y., Ivan C., Kim H.S., Borkowski R.J., Khan S., Rodriguez-Aguayo C., Lopez-Berestein G., Lea J., Gazdar A., Baggerly K.A., Sood A.K. & White M.A.

A genome-scale screen reveals context-dependent ovarian cancer sensitivity to miRNA overexpression

Mol Syst Biol (2015) 11:842.

Pradeep S., et al.

Erythropoietin stimulates tumor growth via EphB4

Cancer Cell (2015) 28:610-22.

Kosutic M., et al.

A mini-Twister variant and impact of residues/cations on the phosphodiester cleavage of this ribozyme class

Angew Chem Int Ed Engl (2015) 54:15128-33.

Nagaraja A.S., et al.

Sustained adrenergic signaling leads to increased metastasis in ovarian cancer via increased PGE2 synthesis

Oncogene (2016) 35:2390-2397.

Ren A., Rajashankar K.R. & Patel D.J.

Global RNA fold and molecular recognition for a pfl riboswitch bound to ZMP, a master regulator of one-carbon metabolism

Structure (2015) 23:1375-81.

Miyake T.M., et al.

XPO1/CRM1 inhibition causes antitumor effects by mitochondrial accumulation of eIF5A

Clin Cancer Res (2015) 21:3286.

Ren A., Wang X.C., Kellenberger C.A., Rajashankar K.R., Jones R.A., Hammond M.C. & Patel D.J.

Structural basis for molecular discrimination by a 3',3'-cGAMP sensing riboswitch

Cell Rep (2015) 11:1-12.

Martinez G.J., et al.

The transcription factor NFAT promotes exhaustion of activated CD8(+) T cells

Immunity (2015) 42:265-78.

Yao C., et al.

Integromic analysis of genetic variation and gene expression identifies networks for cardiovascular disease phenotypes

Circulation (2015) 131:536-49.

Mu J., Zhuang X., Wang Q., Jiang H., Deng Z.B., Wang B., Zhang L., Kakar S., Jun Y., Miller D. & Zhang H.G.

Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles

Mol Nutr Food Res (2014) 58:1561-73.

van d.e.n., Koch E., Chu K., Rupaimoole R., Prickaerts P., Adriaens M., Voncken J.W., Harris A.L., Buffa F.M., Haider S., Starmans M.H., Yao C.Q., Ivan M., Ivan C., Pecot C.V., Boutros P.C., Sood A.K., Koritzinsky M. & Wouters B.G.

Hypoxia promotes stem cell phenotypes and poor prognosis through epigenetic regulation of DICER

Nat Commun (2014) 5:5203. Related Pathway

 

Han C., Liu Y., Wan G., Choi H.J., Zhao L., Ivan C., He X., Sood A.K., Zhang X. & Lu X.

The RNA-binding protein DDX1 promotes primary microRNA maturation and inhibits ovarian tumor progression

Cell Rep (2014) 8:1447-60. Related Pathway

 

Jiang H., Wang P., Li X., Wang Q., Deng Z.B., Zhuang X., Mu J., Zhang L., Wang B., Yan J., Miller D. & Zhang H.G.

Restoration of miR17/20a in solid tumor cells enhances the natural killer cell antitumor activity by targeting Mekk2

Cancer Immunol Res (2014) 2:789-99.

Zhaorigetu S., Rodriguez-Aguayo C., Sood A.K., Lopez-Berestein G. & Walton B.L.

Delivery of negatively charged liposomes into the atherosclerotic plaque of apolipoprotein E-deficient mouse aortic tissue

J Liposome Res (2014) 24:182-90.

Krzeszinski J.Y., Wei W., Huynh H., Jin Z., Wang X., Chang T.C., Xie X.J., He L., Mangala L.S., Lopez-Berestein G., Sood A.K., Mendell J.T. & Wan Y.

miR-34a blocks osteoporosis and bone metastasis by inhibiting osteoclastogenesis and Tgif2

Nature (2014) 512:431-5. Related Pathway

 

Pradeep S., Kim S.W., Wu S.Y., Nishimura M., Chaluvally-Raghavan P., Miyake T., Pecot C.V., Kim S.J., Choi H.J., Bischoff F.Z., Mayer J.A., Huang L., Nick A.M., Hall C.S., Rodriguez-Aguayo C., Zand B., Dalton H.J., Arumugam T., Lee H.J., Han H.D., Cho M.S., Rupaimoole R., Mangala L.S., Sehgal V., Oh S.C., Liu J., Lee J.S., Coleman R.L., Ram P., Lopez-Berestein G., Fidler I.J. & Sood A.K.

Hematogenous metastasis of ovarian cancer: rethinking mode of spread

Cancer Cell (2014) 26:77-91.

Thomas M.F., L'Etoile N.D. & Ansel K.M.

Eri1: a conserved enzyme at the crossroads of multiple RNA-processing pathways

Trends Genet (2014) 30:298-307.

Takahashi K., Yan I.K., Kogure T., Haga H. & Patel T.

Extracellular vesicle-mediated transfer of long non-coding RNA ROR modulates chemosensitivity in human hepatocellular cancer

FEBS Open Bio (2014) 4:458-67.

Takahashi K., Yan I.K., Wood J., Haga H. & Patel T.

Involvement of extracellular vesicle long non-coding RNA (linc-VLDLR) in tumor cell responses to chemotherapy

Mol Cancer Res (2014) 12:1377-87.

Bauer C.A., Kim E.Y., Marangoni F., Carrizosa E., Claudio N.M. & Mempel T.R.

Dynamic Treg interactions with intratumoral APCs promote local CTL dysfunction

J Clin Invest (2014) 124:2425-40.

Hu W., Liu T., Ivan C., Sun Y., Huang J., Mangala L.S., Miyake T., Dalton H.J., Pradeep S., Rupaimoole R., Previs R.A., Han H.D., Bottsford-Miller J., Zand B., Kang Y., Pecot C.V., Nick A.M., Wu S.Y., Lee J.S., Sehgal V., Ram P., Liu J., Tucker S.L., Lopez-Berestein G., Baggerly K.A., Coleman R.L. & Sood A.K.

Notch3 pathway alterations in ovarian cancer

Cancer Res (2014) 74:3282-93. Related Pathway

 

Lindoso R.S., Collino F., Bruno S., Araujo D.S., Sant'Anna J.F., Tetta C., Provero P., Quesenberry P.J., Vieyra A., Einicker-Lamas M. & Camussi G.

Extracellular vesicles released from mesenchymal stromal cells modulate miRNA in renal tubular cells and inhibit ATP depletion injury

Stem Cells Dev (2014) 23:1809-19.

Takahashi K., Yan I.K., Haga H. & Patel T.

Modulation of hypoxia-signaling pathways by extracellular linc-RoR

J Cell Sci (2014) 127:1585-94.

Beaulieu L.M., Lin E., Mick E., Koupenova M., Weinberg E.O., Kramer C.D., Genco C.A., Tanriverdi K., Larson M.G., Benjamin E.J. & Freedman J.E.

Interleukin 1 receptor 1 and interleukin 1beta regulate megakaryocyte maturation, platelet activation, and transcript profile during inflammation in mice and humans

Arterioscler Thromb Vasc Biol (2014) 34:552-64. Related Pathway

 

Ren A., Kosutic M., Rajashankar K.R., Frener M., Santner T., Westhof E., Micura R. & Patel D.J.

In-line alignment and Mg(2)(+) coordination at the cleavage site of the env22 twister ribozyme

Nat Commun (2014) 5:5534.

Ren A. & Patel D.J.

c-di-AMP binds the ydaO riboswitch in two pseudo-symmetry-related pockets

Nat Chem Biol (2014) 10:780-6.

Pusic K.M., Pusic A.D., Kemme J. & Kraig R.P.

Spreading depression requires microglia and is decreased by their M2a polarization from Environmental Enrichment

Glia (2014) 62:1176-94.

Beaulieu L.M., et al.

Interleukin 1 receptor 1 and interleukin 1β regulate megakaryocyte maturation, platelet activation, and transcript profile during inflammation in mice and humans

Arterioscler Thromb Vasc Biol (2014) 34:552-64.

Panagopoulos K., Cross-Knorr S., Dillard C., Pantazatos D., Del T.a.t.t.o., Mills D., Goldstein L., Renzulli J., Quesenberry P. & Chatterjee D.

Reversal of chemosensitivity and induction of cell malignancy of a non-malignant prostate cancer cell line upon extracellular vesicle exposure

Mol Cancer (2013) 12:118.

Aliotta J.M., Pereira M., Amaral A., Sorokina A., Igbinoba Z., Hasslinger A., El-Bizri R., Rounds S.I., Quesenberry P.J. & Klinger J.R.

Induction of pulmonary hypertensive changes by extracellular vesicles from monocrotaline-treated mice

Cardiovasc Res (2013) 100:354-62.

Deng Z.B., Zhuang X., Ju S., Xiang X., Mu J., Liu Y., Jiang H., Zhang L., Mobley J., McClain C., Feng W., Grizzle W., Yan J., Miller D., Kronenberg M. & Zhang H.G.

Exosome-like nanoparticles from intestinal mucosal cells carry prostaglandin E2 and suppress activation of liver NKT cells

J Immunol (2013) 190:3579-89.

Nishimura M., Jung E.J., Shah M.Y., Lu C., Spizzo R., Shimizu M., Han H.D., Ivan C., Rossi S., Zhang X., Nicoloso M.S., Wu S.Y., Almeida M.I., Bottsford-Miller J., Pecot C.V., Zand B., Matsuo K., Shahzad M.M., Jennings N.B., Rodriguez-Aguayo C., Lopez-Berestein G., Sood A.K. & Calin G.A.

Therapeutic synergy between microRNA and siRNA in ovarian cancer treatment

Cancer Discov (2013) 3:1302-15.

Quesenberry P.J., Dooner M.S., Goldberg L.R., Aliotta J.M., Pereira M., Amaral A., Del T.a.t.t.o., Hixson D.C. & Ramratnam B.

A new stem cell biology: the continuum and microvesicles

Trans Am Clin Climatol Assoc (2012) 123:152-66; discussion 166.

Biomarkers

Gladek I., Ferdin J., Horvat S., Calin G.A. & Kunej T.

HIF1A gene polymorphisms and human diseases: Graphical review of 97 association studies

Genes Chromosomes Cancer AOP 2017-02-06.

McManus D.D., et al.

Messenger RNA and microRNA transcriptomic signatures of cardiometabolic risk factors

BMC Genomics (2017) 18:139.

Moustafa A.A., et al.

Identification of microRNA signature and potential pathway targets in prostate cancer

Exp Biol Med (Maywood) (2017) 242:536-546.

Dou Y., et al.

Circular RNAs are down-regulated in KRAS mutant colon cancer cells and can be transferred to exosomes

Sci Rep (2016) 6:37982.

Chaluvally-Raghavan P., et al.

Direct upregulation of STAT3 by microRNA-551b-3p deregulates growth and metastasis of ovarian cancer

Cell Rep (2016) 15:1493-504.

Shah R., Tanriverdi K., Levy D., Larson M., Gerstein M., Mick E., Rozowsky J., Kitchen R., Murthy V., Mikalev E. & Freedman J.E.

Discordant Expression of Circulating microRNA from Cellular and Extracellular Sources

PLoS One (2016) 11:e0153691.

Freedman J.E., et al.

Diverse human extracellular RNAs are widely detected in human plasma

Nat Commun (2016) 7:11106.

Rennert R.C., Hochberg F.H. & Carter B.S.

ExRNA in biofluids as biomarkers for brain tumors

Cell Mol Neurobiol (2016) 36:353-60.

Mohankumar S. & Patel T.

Extracellular vesicle long noncoding RNA as potential biomarkers of liver cancer

Brief Funct Genomics (2016) 15:249-56.

Fabris L., Ceder Y., Chinnaiyan A.M., Jenster G.W., Sorensen K.D., Tomlins S., Visakorpi T. & Calin G.A.

The potential of microRNAs as prostate cancer biomarkers

Eur Urol AOP 2016-01-21.

Ben-Dov I.Z., Whalen V.M., Goilav B., Max K.E. & Tuschl T.

Cell and microvesicle urine microRNA deep sequencing profiles from healthy individuals: Observations with potential impact on biomarker studies

PLoS ONE (2016) 11:e0147249.

Quinn J.F., Patel T., Wong D., Das S., Freedman J.E., Laurent L.C., Carter B.S., Hochberg F., Van K.e.u.r.e.n.-.J.e.n.s.e.n., Huentelman M., Spetzler R., Kalani M.Y., Arango J., Adelson P.D., Weiner H.L., Gandhi R., Goilav B., Putterman C. & Saugstad J.A.

Extracellular RNAs: development as biomarkers of human disease

J Extracell Vesicles (2015) 4:27495.

Lin X., Lo H.C., Wong D.T. & Xiao X.

Noncoding RNAs in human saliva as potential disease biomarkers

Front Genet (2015) 6:175.

Melman Y.F., Shah R., Danielson K., Xiao J., Simonson B., Barth A., Chakir K., Lewis G.D., Lavender Z., Truong Q.A., Kleber A., Das R., Rosenzweig A., Wang Y., Kass D.A., Singh J.P. & Das S.

Circulating microRNA-30d is associated with response to cardiac resynchronization therapy in heart failure and regulates cardiomyocyte apoptosis: A translational pilot study

Circulation (2015) 131:2202-16.

Melman Y.F., Shah R., Danielson K., Xiao J., Simonson B., Barth A., Chakir K., Lewis G.D., Lavender Z., Truong Q.A., Kleber A., Das R., Rosenzweig A., Wang Y., Kass D.A., Singh J.P. & Das S.

Response to letter regarding article, "Circulating microRNA-30d is associated with response to cardiac resynchronization therapy in heart failure and regulates cardiomyocyte apoptosis: A translational pilot study"

Circulation (2016) 133:e389-90.

Akers J.C., Ramakrishnan V., Kim R., Phillips S., Kaimal V., Mao Y., Hua W., Yang I., Fu C.C., Nolan J., Nakano I., Yang Y., Beaulieu M., Carter B.S. & Chen C.C.

miRNA contents of cerebrospinal fluid extracellular vesicles in glioblastoma patients

J Neurooncol (2015) 123:205-16.

Majem B., Rigau M., Reventos J. & Wong D.T.

Non-coding RNAs in saliva: emerging biomarkers for molecular diagnostics

Int J Mol Sci (2015) 16:8676-8698.

Ling H., Pickard K., Ivan C., Isella C., Ikuo M., Mitter R., Spizzo R., Bullock M.D., Braicu C., Pileczki V., Vincent K., Pichler M., Stiegelbauer V., Hoefler G., Almeida M.I., Hsiao A., Zhang X., Primrose J.N., Packham G.K., Liu K., Bojja K., Gafa R., Xiao L., Rossi S., Song J.H., Vannini I., Fanini F., Kopetz S., Zweidler-McKay P., Wang X., Ionescu C., Irimie A., Fabbri M., Lanza G., Hamilton S.R., Berindan-Neagoe I., Medico E., Mirnezami A.H., Calin G.A. & Nicoloso M.S.

The clinical and biological significance of MIR-224 expression in colorectal cancer metastasis

Gut (2016) 65:977-989.

Ho G.Y., Jung H.J., Schoen R.E., Wang T., Lin J., Williams Z., Weissfeld J.L., Park J.Y., Loudig O. & Suh Y.

Differential expression of circulating microRNAs according to severity of colorectal neoplasia

Transl Res (2015) 166:225-32.

Boukouris S. & Mathivanan S.

Exosomes in bodily fluids are a highly stable resource of disease biomarkers

Proteomics Clin Appl (2015) 9:358-67.

Wong D.T.

Salivary extracellular noncoding RNA: Emerging biomarkers for molecular diagnostics

Clin Ther (2015) 37:540-51.

Fuentes L., Yakob M. & Wong D.T.

Emerging horizons of salivary diagnostics for periodontal disease

Br Dent J (2014) 217:567-73.

Bahn J.H., Zhang Q., Li F., Chan T.M., Lin X., Kim Y., Wong D.T. & Xiao X.

The landscape of microRNA, Piwi-interacting RNA, and circular RNA in human saliva

Clin Chem (2015) 61:221-30.

Haga H. & Patel T.

Molecular diagnosis of intrahepatic cholangiocarcinoma

J Hepatobiliary Pancreat Sci (2015) 22:114-23.

Shaikh A.Y., et al.

Addition of B-type natriuretic peptide to existing clinical risk scores enhances identification of patients at risk for atrial fibrillation recurrence after pulmonary vein isolation

Crit Pathw Cardiol (2015) 14:157-65.

Aslan B., et al.

The ZNF304-integrin axis protects against anoikis in cancer

Nat Commun (2015) 6:7351.

Kwong R.Y., Heydari B., Abbasi S., Steel K., Al-Mallah M., Wu H. & Falk R.H.

Characterization of cardiac amyloidosis by atrial late gadolinium enhancement using contrast-enhanced cardiac magnetic resonance imaging and correlation with left atrial conduit and contractile function

Am J Cardiol (2015) 116:622-9.

Koupenova M., Mick E., Mikhalev E., Benjamin E.J., Tanriverdi K. & Freedman J.E.

Sex differences in platelet Toll-like receptors and their association with cardiovascular risk factors

Arterioscler Thromb Vasc Biol (2015) 35:1030-7.

Akat K.M., Moore-McGriff D., Morozov P., Brown M., Gogakos T., Correa D.a., Mihailovic A., Sauer M., Ji R., Ramarathnam A., Totary-Jain H., Williams Z., Tuschl T. & Schulze P.C.

Comparative RNA-sequencing analysis of myocardial and circulating small RNAs in human heart failure and their utility as biomarkers

Proc Natl Acad Sci U S A (2014) 111:11151-6.

Yakob M., Fuentes L., Wang M.B., Abemayor E. & Wong D.T.

Salivary biomarkers for detection of oral squamous cell carcinoma - current state and recent advances

Curr Oral Health Rep (2014) 1:133-141.

Yang L., Moss T., Mangala L.S., Marini J., Zhao H., Wahlig S., Armaiz-Pena G., Jiang D., Achreja A., Win J., Roopaimoole R., Rodriguez-Aguayo C., Mercado-Uribe I., Lopez-Berestein G., Liu J., Tsukamoto T., Sood A.K., Ram P.T. & Nagrath D.

Metabolic shifts toward glutamine regulate tumor growth, invasion and bioenergetics in ovarian cancer

Mol Syst Biol (2014) 10:728. Related Pathway

 

Tucker S.L., Gharpure K., Herbrich S.M., Unruh A.K., Nick A.M., Crane E.K., Coleman R.L., Guenthoer J., Dalton H.J., Wu S.Y., Rupaimoole R., Lopez-Berestein G., Ozpolat B., Ivan C., Hu W., Baggerly K.A. & Sood A.K.

Molecular biomarkers of residual disease after surgical debulking of high-grade serous ovarian cancer

Clin Cancer Res (2014) 20:3280-8.

Hochberg F.H., Atai N.A., Gonda D., Hughes M.S., Mawejje B., Balaj L. & Carter R.S.

Glioma diagnostics and biomarkers: an ongoing challenge in the field of medicine and science

Expert Rev Mol Diagn (2014) 14:439-52.

Ben-Dov I.Z., Tan Y.C., Morozov P., Wilson P.D., Rennert H., Blumenfeld J.D. & Tuschl T.

Urine microRNA as potential biomarkers of autosomal dominant polycystic kidney disease progression: description of miRNA profiles at baseline

PLoS ONE (2014) 9:e86856.

McManus D.D., Lin H., Tanriverdi K., Quercio M., Yin X., Larson M.G., Ellinor P.T., Levy D., Freedman J.E. & Benjamin E.J.

Relations between circulating microRNAs and atrial fibrillation: data from the Framingham Offspring Study

Heart Rhythm (2014) 11:663-9.

Ngamruengphong S. & Patel T.

Molecular evolution of genetic susceptibility to hepatocellular carcinoma

Dig Dis Sci (2014) 59:986-91.

Akers J.C., Ramakrishnan V., Kim R., Skog J., Nakano I., Pingle S., Kalinina J., Hua W., Kesari S., Mao Y., Breakefield X.O., Hochberg F.H., Van M.e.i.r., Carter B.S. & Chen C.C.

MiR-21 in the extracellular vesicles (EVs) of cerebrospinal fluid (CSF): a platform for glioblastoma biomarker development

PLoS ONE (2013) 8:e78115.

Lau C., Kim Y., Chia D., Spielmann N., Eibl G., Elashoff D., Wei F., Lin Y.L., Moro A., Grogan T., Chiang S., Feinstein E., Schafer C., Farrell J. & Wong D.T.

Role of pancreatic cancer-derived exosomes in salivary biomarker development

J Biol Chem (2013) 288:26888-97.

Therapies

Haga H., Yan I.K., Takahashi K., Matsuda A. & Patel T.

Extracellular vesicles from bone marrow-derived mesenchymal stem cells improve survival from lethal hepatic failure in mice

Stem Cells Transl Med AOP 2017-02-18.

Seviour E.G., et al.

Targeting KRas-dependent tumour growth, circulating tumour cells and metastasis in vivo by clinically significant miR-193a-3p

Oncogene (2017) 36:1339-1350.

Harjes U., et al.

Antiangiogenic and tumour inhibitory effects of downregulating tumour endothelial FABP4

Oncogene (2017) 36:912-921.

Heydari B., Harris W.S. & Kwong R.Y.

Response by Heydari et al. to Letter regarding Article, "Effect of Omega-3 acid ethyl esters on left ventricular remodeling after acute myocardial infarction: The OMEGA-REMODEL randomized clinical trial"

Circulation (2017) 135:e13-e14.

Van Roosbroeck K., et al.

Combining anti-miR-155 with chemotherapy for the treatment of lung cancers

Clin Cancer Res AOP 2016-11-30.

Wen S., et al.

Mesenchymal stromal cell-derived extracellular vesicles rescue radiation damage to murine marrow hematopoietic cells

Leukemia AOP 2016-05-27.

Rosas L.E., Elgamal O.A., Mo X., Phelps M.A., Schmittgen T.D. & Papenfuss T.L.

In vitro immunotoxicity assessment of culture-derived extracellular vesicles in human monocytes

J Immunotoxicol AOP 2016-04-14.

Huang J., Hu W., Hu L., Previs R.A., Dalton H.J., Yang X.Y., Sun Y., McGuire M., Rupaimoole R., Nagaraja A.S., Kang Y., Liu T., Nick A.M., Jennings N.B., Coleman R.L., Jaffe R.B. & Sood A.K.

Dll4 inhibition plus Aflibercept markedly reduces ovarian tumor growth

Mol Cancer Ther AOP 2016-03-23.

Heydari B., et al.

Effect of Omega-3 acid ethyl esters on left ventricular remodeling after acute myocardial infarction: The OMEGA-REMODEL randomized clinical trial

Circulation (2016) 134:378-91.

Hatakeyama H., et al.

Role of CTGF in sensitivity to hyperthermia in ovarian and uterine cancers

Cell Rep (2016) 17:1621-1631.

Ohtsuka M., et al.

H19 noncoding RNA, an independent prognostic factor, regulates essential Rb-E2F and CDK8-beta-catenin signaling in colorectal cancer

EBioMedicine (2016) 13:113-124.

Mangala L.S., et al.

Improving vascular maturation using noncoding RNAs increases antitumor effect of chemotherapy

JCI Insight (2016) 1:e87754.

Shah M.Y., Ferrajoli A., Sood A.K., Lopez-Berestein G. & Calin G.A.

microRNA therapeutics in cancer - An emerging concept

EBioMedicine (2016) 12:34-42.

Tao L., et al.

Crucial role of miR-433 in regulating cardiac fibrosis

Theranostics (2016) 6:2068-2083.

Pan Y., et al.

miR-509-3p is clinically significant and strongly attenuates cellular migration and multi-cellular spheroids in ovarian cancer

Oncotarget (2016) 7:25930-48.

Teng Y., et al.

Grapefruit-derived nanovectors deliver miR-18a for treatment of liver metastasis of colon cancer by induction of M1 macrophages

Oncotarget (2016) 7:25683-97.

Hatakeyama H., Wu S.Y., Mangala L.S., Lopez-Berestein G. & Sood A.K.

Assessment of in vivo siRNA delivery in cancer mouse models

Methods Mol Biol (2016) 1402:189-97.

Chaluvally-Raghavan P., et al.

Direct upregulation of STAT3 by microRNA-551b-3p deregulates growth and metastasis of ovarian cancer

Cell Rep (2016) 15:1493-504.

Hall J., Prabhakar S., Balaj L., Lai C.P., Cerione R.A. & Breakefield X.O.

Delivery of therapeutic proteins via extracellular vesicles: Review and potential treatments for Parkinson's disease, glioma, and Schwannoma

Cell Mol Neurobiol (2016) 36:417-27.

Parasramka M.A., Maji S., Matsuda A., Yan I.K. & Patel T.

Long non-coding RNAs as novel targets for therapy in hepatocellular carcinoma

Pharmacol Ther (2016) 161:67-78. Related Pathway

 

Kalani M.Y., Siniard A.L., Corneveaux J.J., Bruhns R., Richholt R., Forseth J., Zabramski J.M., Nakaji P., Spetzler R.F. & Huentelman M.J.

Rare variants in cardiomyopathy genes associated with stress-induced cardiomyopathy

Neurosurgery (2016) 78:835-43.

Byron S.A., Van Keuren-Jensen K.R., Engelthaler D.M., Carpten J.D. & Craig D.W.

Translating RNA sequencing into clinical diagnostics: Opportunities and challenges

Nat Rev Genet (2016) 17:257-271. Related blog post

Pusic K.M., Pusic A.D. & Kraig R.P.

Environmental Enrichment stimulates immune cell secretion of exosomes that promote CNS myelination and may regulate inflammation

Cell Mol Neurobiol (2016) 36:313-325.

Rupaimoole R., Calin G.A., Lopez-Berestein G. & Sood A.K.

miRNA deregulation in cancer cells and the tumor microenvironment

Cancer Discov (2016) 6:235-246.

Mitamura T., Gourley C. & Sood A.K.

Prediction of anti-angiogenesis escape

Gynecol Oncol (2016) 141:80-85.

Wang J.Q., et al.

Extracellular vesicle-mediated reversal of Paclitaxel resistance in prostate cancer

Crit Rev Oncog (2015) 20:407-17.

Reyes-Gonzalez J.M., Armaiz-Pena G.N., Mangala L.S., Valiyeva F., Ivan C., Pradeep S., Echevarria-Vargas I.M., Rivera-Reyes A., Sood A.K. & Vivas-Mejia P.E.

Targeting c-MYC in platinum-resistant ovarian cancer

Mol Cancer Ther (2015) 14:2260-9.

Pusic A.D. & Kraig R.P.

Phasic treatment with Interferon Gamma stimulates release of exosomes that protect against spreading depression

J Interferon Cytokine Res (2015) 35:795-807.

Zhuang X., Teng Y., Samykutty A., Mu J., Deng Z., Zhang L., Cao P., Rong Y., Yan J., Miller D. & Zhang H.G.

Grapefruit-derived nanovectors delivering therapeutic miR17 through an intranasal route inhibit brain tumor progression

Mol Ther (2016) 24:96-105.

Quesenberry P.J., Aliotta J., Camussi G., Abdel-Mageed A.B., Wen S., Goldberg L., Zhang H.G., Tetta C., Franklin J., Coffey R.J., Danielson K., Subramanya V., Ghiran I., Das S., Chen C.C., Pusic K.M., Pusic A.D., Chatterjee D., Kraig R.P., Balaj L. & Dooner M.

Potential functional applications of extracellular vesicles: a report by the NIH Common Fund Extracellular RNA Communication Consortium

J Extracell Vesicles (2015) 4:27575.

Monroig P.d.e.l., Chen L., Zhang S. & Calin G.A.

Small molecule compounds targeting miRNAs for cancer therapy

Adv Drug Deliv Rev (2015) 81:104-16.

Wang Q., Ren Y., Mu J., Egilmez N.K., Zhuang X., Deng Z., Zhang L., Yan J., Miller D. & Zhang H.G.

Grapefruit-derived nanovectors use an activated leukocyte trafficking pathway to deliver therapeutic agents to inflammatory tumor sites

Cancer Res (2015) 75:2520-2529.

Simonson B. & Das S.

MicroRNA therapeutics: the next magic bullet?

Mini Rev Med Chem (2015) 15:467-74.

Ozcan G., Ozpolat B., Coleman R.L., Sood A.K. & Lopez-Berestein G.

Preclinical and clinical development of siRNA-based therapeutics

Adv Drug Deliv Rev (2015) 87:108–119.

George J. & Patel T.

Noncoding RNA as therapeutic targets for hepatocellular carcinoma

Semin Liver Dis (2015) 35:63-74.

Gyorgy B., Hung M.E., Breakefield X.O. & Leonard J.N.

Therapeutic applications of extracellular vesicles: clinical promise and open questions

Annu Rev Pharmacol Toxicol (2015) 55:439-64.

Previs R.A., et al.

Dual metronomic chemotherapy with Nab-Paclitaxel and Topotecan has potent antiangiogenic activity in ovarian cancer

Mol Cancer Ther (2015) 14:2677-86.

Gharpure K.M., Wu S.Y., Li C., Lopez-Berestein G. & Sood A.K.

Nanotechnology: Future of oncotherapy

Clin Cancer Res (2015) 21:3121-30.

Aslan B., et al.

The ZNF304-integrin axis protects against anoikis in cancer

Nat Commun (2015) 6:7351.

Miyake T.M., et al.

XPO1/CRM1 inhibition causes antitumor effects by mitochondrial accumulation of eIF5A

Clin Cancer Res (2015) 21:3286.

Thanapprapa D., et al.

PTEN expression as a predictor of response to focal adhesion kinase inhibition in uterine cancer

Mol Cancer Ther (2015) 14:1466.

Nick A.M., Coleman R.L., Ramirez P.T. & Sood A.K.

A framework for a personalized surgical approach to ovarian cancer

Nat Rev Clin Oncol (2015) 12:239-45.

Gonzalez-Villasana V., et al.

Rac1/Pak1/p38/MMP-2 axis regulates angiogenesis in ovarian cancer

Clin Cancer Res (2015) 21:2127-37.

Choi H.J., Armaiz P.e.n.a., Pradeep S., Cho M.S., Coleman R.L. & Sood A.K.

Anti-vascular therapies in ovarian cancer: Moving beyond anti-VEGF approaches

Cancer Metastasis Rev (2015) 34:19-40.

Yang X., Shen F., Hu W., Coleman R.L. & Sood A.K.

New ways to successfully target tumor vasculature in ovarian cancer

Curr Opin Obstet Gynecol (2015) 27:58-65.

Pusic A.D., Mitchell H.M., Kunkler P.E., Klauer N. & Kraig R.P.

Spreading depression transiently disrupts myelin via interferon-gamma signaling

Exp Neurol (2015) 264:43-54.

Bottsford-Miller J., et al.

Differential platelet levels affect response to taxane-based therapy in ovarian cancer

Clin Cancer Res (2015) 21:602-10.

Wen Y., et al.

Immunotherapy targeting folate receptor induces cell death associated with autophagy in ovarian cancer

Clin Cancer Res (2015) 21:448-59.

Previs R.A., Coleman R.L., Harris A.L. & Sood A.K.

Molecular pathways: Translational and therapeutic implications of the Notch signaling pathway in cancer

Clin Cancer Res (2015) 21:955-61.

Patel T. & Harnois D.

Assessment of response to therapy in hepatocellular carcinoma

Ann Med (2014) 46:130-7.

Leung C.S., Yeung T.L., Yip K.P., Pradeep S., Balasubramanian L., Liu J., Wong K.K., Mangala L.S., Armaiz-Pena G.N., Lopez-Berestein G., Sood A.K., Birrer M.J. & Mok S.C.

Calcium-dependent FAK/CREB/TNNC1 signalling mediates the effect of stromal MFAP5 on ovarian cancer metastatic potential

Nat Commun (2014) 5:5092. Related Pathway

 

Wu S.Y., Lopez-Berestein G., Calin G.A. & Sood A.K.

RNAi therapies: drugging the undruggable

Sci Transl Med (2014) 6:240ps7.

Gharpure K.M., Chu K.S., Bowerman C.J., Miyake T., Pradeep S., Mangala S.L., Han H.D., Rupaimoole R., Armaiz-Pena G.N., Rahhal T.B., Wu S.Y., Luft J.C., Napier M.E., Lopez-Berestein G., DeSimone J.M. & Sood A.K.

Metronomic docetaxel in PRINT nanoparticles and EZH2 silencing have synergistic antitumor effect in ovarian cancer

Mol Cancer Ther (2014) 13:1750-7.

Patel T. & Harnois D.

Assessment of response to therapy in hepatocellular carcinoma

Ann Med (2014) 46:130-7.

Vader P., Breakefield X.O. & Wood M.J.

Extracellular vesicles: emerging targets for cancer therapy

Trends Mol Med (2014) 20:385-393.

Pusic A.D., Pusic K.M. & Kraig R.P.

What are exosomes and how can they be used in multiple sclerosis therapy?

Expert Rev Neurother (2014) 14:353-5.

Pusic A.D., Pusic K.M., Clayton B.L. & Kraig R.P.

IFNgamma-stimulated dendritic cell exosomes as a potential therapeutic for remyelination

J Neuroimmunol (2014) 266:12-23.

Gonzalez-Villasana V., et al.

Bisphosphonates inhibit stellate cell activity and enhance antitumor effects of nanoparticle albumin-bound Paclitaxel in pancreatic ductal adenocarcinoma

Mol Cancer Ther (2014) 13:2583-94.

Patel T. & Harnois D.

Assessment of response to therapy in hepatocellular carcinoma

Ann Med (2014) 46:130-7.

Zhang H., et al.

Patient-derived xenografts of triple-negative breast cancer reproduce molecular features of patient tumors and respond to mTOR inhibition

Breast Cancer Res (2014) 16:R36.

Jun H.J., Bronson R.T. & Charest A.

Inhibition of EGFR induces a c-MET-driven stem cell population in glioblastoma

Stem Cells (2014) 32:338-48.

Camussi G. & Quesenberry P.J.

Perspectives on the potential therapeutic uses of vesicles

Exosomes Microvesicles (2013) 1:6.

Wang Q., Zhuang X., Mu J., Deng Z.B., Jiang H., Zhang L., Xiang X., Wang B., Yan J., Miller D. & Zhang H.G.

Delivery of therapeutic agents by nanoparticles made of grapefruit-derived lipids

Nat Commun (2013) 4:1867.

Pecot C.V., Rupaimoole R., Yang D., Akbani R., Ivan C., Lu C., Wu S., Han H.D., Shah M.Y., Rodriguez-Aguayo C., Bottsford-Miller J., Liu Y., Kim S.B., Unruh A., Gonzalez-Villasana V., Huang L., Zand B., Moreno-Smith M., Mangala L.S., Taylor M., Dalton H.J., Sehgal V., Wen Y., Kang Y., Baggerly K.A., Lee J.S., Ram P.T., Ravoori M.K., Kundra V., Zhang X., Ali-Fehmi R., Gonzalez-Angulo A.M., Massion P.P., Calin G.A., Lopez-Berestein G., Zhang W. & Sood A.K.

Tumour angiogenesis regulation by the miR-200 family

Nat Commun (2013) 4:2427.

Nishimura M., Jung E.J., Shah M.Y., Lu C., Spizzo R., Shimizu M., Han H.D., Ivan C., Rossi S., Zhang X., Nicoloso M.S., Wu S.Y., Almeida M.I., Bottsford-Miller J., Pecot C.V., Zand B., Matsuo K., Shahzad M.M., Jennings N.B., Rodriguez-Aguayo C., Lopez-Berestein G., Sood A.K. & Calin G.A.

Therapeutic synergy between microRNA and siRNA in ovarian cancer treatment

Cancer Discov (2013) 3:1302-15.

Methods

Gladek I., Ferdin J., Horvat S., Calin G.A. & Kunej T.

HIF1A gene polymorphisms and human diseases: Graphical review of 97 association studies

Genes Chromosomes Cancer AOP 2017-02-06.

Majem B., Li F., Sun J. & Wong D.T.

RNA sequencing analysis of salivary extracellular RNA

Methods Mol Biol (2017) 1537:17-36.

Lee I., Baxter D., Lee M.Y., Scherler K. & Wang K.

The importance of standardization on analyzing circulating RNA

Mol Diagn Ther AOP 2016-12-30.

Freedman J.E., et al.

Diverse human extracellular RNAs are widely detected in human plasma

Nat Commun (2016) 7:11106.

Cheung K.H., et al.

Extending gene ontology in the context of extracellular RNA and vesicle communication

J Biomed Semantics (2016) 7:19.

Clarke D., Sethi A., Li S., Kumar S., Chang R.W., Chen J. & Gerstein M.

Identifying allosteric hotspots with dynamics: Application to inter- and intra-species conservation

Structure (2016) 24:826-37.

Tanriverdi K., Kucukural A., Mikhalev E., Tanriverdi S.E., Lee R., Ambros V.R. & Freedman J.E.

Comparison of RNA isolation and associated methods for extracellular RNA detection by high-throughput quantitative Polymerase Chain Reaction

Anal Biochem (2016) 501:66-74.

Akers J.C., Ramakrishnan V., Nolan J.P., Duggan E., Fu C.C., Hochberg F.H., Chen C.C. & Carter B.S.

Comparative analysis of technologies for quantifying extracellular vesicles (EVs) in clinical cerebrospinal fluids (CSF)

PLoS ONE (2016) 11:e0149866.

Danielson K.M., Estanislau J., Tigges J., Toxavidis V., Camacho V., Felton E.J., Khoory J., Kreimer S., Ivanov A.R., Mantel P.Y., Jones J., Akuthota P., Das S. & Ghiran I.

Diurnal variations of circulating extracellular vesicles measured by nano flow cytometry

PLoS ONE (2016) 11:e0144678.

Keerthikumar S., Chisanga D., Ariyaratne D., Al Saffar H., Anand S., Zhao K., Samuel M., Pathan M., Jois M., Chilamkurti N., Gangoda L. & Mathivanan S.

ExoCarta: A web-based compendium of exosomal cargo

J Mol Biol (2016) 428:688-692.

Laurent L.C., Abdel-Mageed A.B., Adelson P.D., Arango J., Balaj L., Breakefield X., Carlson E., Carter B.S., Majem B., Chen C.C., Cocucci E., Danielson K., Courtright A., Das S., Abd E.l.m.a.g.e.e.d., Enderle D., Ezrin A., Ferrer M., Freedman J., Galas D., Gandhi R., Huentelman M.J., Van Keuren-Jensen K., Kalani Y., Kim Y., Krichevsky A.M., Lai C., Lal-Nag M., Laurent C.D., Leonardo T., Li F., Malenica I., Mondal D., Nejad P., Patel T., Raffai R.L., Rubio R., Skog J., Spetzler R., Sun J., Tanriverdi K., Vickers K., Wang L., Wang Y., Wei Z., Weiner H.L., Wong D., Yan I.K., Yeri A. & Gould S.

Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium

J Extracell Vesicles (2015) 4:26533.

Subramanian S.L., Kitchen R.R., Alexander R.P., Carter B.S., Cheung K.H., Laurent L.C., Pico A., Roberts L.R., Roth M.E., Rozowsky J.S., Su A.I., Gerstein M.B. & Milosavljevic A.

Integration of extracellular RNA profiling data using metadata, biomedical ontologies and Linked Data technologies

J Extracell Vesicles (2015) 4:27497.

Chiang S.H., Thomas G.A., Liao W., Grogan T., Buck R.L., Fuentes L., Yakob M., Laughlin M.J., Schafer C., Nazmul-Hossain A., Wei F., Elashoff D., Slowey P.D. & Wong D.T.

RNAPro*SAL: a device for rapid and standardized collection of saliva RNA and proteins

Biotechniques (2015) 58:69-76.

Li X., Mauro M. & Williams Z.

Comparison of plasma extracellular RNA isolation kits reveals kit-dependent biases

Biotechniques (2015) 59:13-17.

Szabo L., Morey R., Palpant N.J., Wang P.L., Afari N., Jiang C., Parast M.M., Murry C.E., Laurent L.C. & Salzman J.

Statistically based splicing detection reveals neural enrichment and tissue-specific induction of circular RNA during human fetal development

Genome Biol (2015) 16:126.

Boettcher M. & McManus M.T.

Choosing the right tool for the job: RNAi, TALEN, or CRISPR

Mol Cell (2015) 58:575-85.

Lai C.P., Kim E.Y., Badr C.E., Weissleder R., Mempel T.R., Tannous B.A. & Breakefield X.O.

Visualization and tracking of tumour extracellular vesicle delivery and RNA translation using multiplexed reporters

Nat Commun (2015) 6:7029.

Shao H., Chung J., Lee K., Balaj L., Min C., Carter B.S., Hochberg F.H., Breakefield X.O., Lee H. & Weissleder R.

Chip-based analysis of exosomal mRNA mediating drug resistance in glioblastoma

Nat Commun (2015) 6:6999.

Li X., Ben-Dov I.Z., Mauro M. & Williams Z.

Lowering the quantification limit of the QubitTM RNA HS assay using RNA spike-in

BMC Mol Biol (2015) 16:9.

Ong W.Q., Citron Y.R., Schnitzbauer J., Kamiyama D. & Huang B.

Heavy water: a simple solution to increasing the brightness of fluorescent proteins in super-resolution imaging

Chem Commun (2015) 51:13451-3.

Lotvall J., Hill A.F., Hochberg F., Buzas E.I., Di V.i.z.i.o., Gardiner C., Gho Y.S., Kurochkin I.V., Mathivanan S., Quesenberry P., Sahoo S., Tahara H., Wauben M.H., Witwer K.W. & Thery C.

Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles

J Extracell Vesicles (2014) 3:26913.

Zhang H., et al.

Patient-derived xenografts of triple-negative breast cancer reproduce molecular features of patient tumors and respond to mTOR inhibition

Breast Cancer Res (2014) 16:R36.

Takahashi K., Yan I.K., Kim C., Kim J. & Patel T.

Analysis of extracellular RNA by digital PCR

Front Oncol (2014) 4:129.

pre-ERCC Bibliography

Papers Related to exRNA

This is not a comprehensive list, but a snapshot to serve as a guide to the published literature.  There are several other relevant published studies.   Inclusion in this list, or omission from this list of any published work does not indicate impact or significance.

Categories

  • Reviews

  • Observations/Biomarkers

  • Mechanisms & Models

  • Methods/Protocols

Reviews

Cocucci, E., Racchetti, G. & Meldolesi, J. Shedding microvesicles: artefacts no more. Trends Cell Biol 19, 43-51, doi:10.1016/J.Tcb.2008.11.003 (2009).

Etheridge, A., Gomes, Clarissa P. C., Pereira, R.W., Galas, D.J., & Wang, K. 2013. The complexity, function, and applications of RNA in circulation. Front. Genet., doi: 10.3389/fgene.2013.00115.

Fabian, M. R., Sonenberg, N. & Filipowicz, W. 2010. Regulation of mRNA translation and stability by microRNAs. Annu Rev Biochem, 79, 351-79.

Gerdes, H. H. & Carvalho, R.N. 2008. Intercellular transfer mediated by tunneling nanotubes. Curr Opin Cell Biol, 20, 470-5.

Kulp, A., & Kuehn, M.J. 2010. Biological Functions and Biogenesis of Secreted Bacterial Outer Membrane Vesicles. Annual Review of Microbiology, 64(1), 163–184.

Lo, Y. M. & Chiu, R.W. 2012. Genomic analysis of fetal nucleic acids in maternal blood. Annu Rev Genomics Hum Genet, 13, 285-306.

Lotvall, J. & Valadi, H. 2007. Cell to cell signalling via exosomes through esRNA. Cell Adh Migr, 1, 156-8.

Mause, S. F. & Weber, C. Microparticles: Protagonists of a Novel Communication Network for Intercellular Information Exchange. Circ Res 107, 1047-1057, doi:10.1161/Circresaha.110.226456 (2010).

Repoila, F., & Darfeuille, F. 2009. Small regulatory non-coding RNAs in bacteria: physiology and mechanistic aspects. Biology of the Cell, 101(2), 117–131.

Shifrin, D.A., Beckler, M.D., Coffey, R.J. & Tyska, M.J. Extracellular vesicles: communication, coercion, and conditioning. Mol Biol Cell 24, 1253-1259, doi:10.1091/Mbc.E12-08-0572 (2013).

Vogel, J., & Wagner, E.G.H. 2007. Target identification of small noncoding RNAs in bacteria. Current Opinion in Microbiology, 10(3), 262–270.

Observations / Biomarkers

Arroyo, J.D., Chevillet, J.R., Kroh, E.M., Ruf, I.K., Pritchard, C.C., Gibson, D.F., Mitchell, P.S., Bennett, C.F., Pogosova-Agadjanyan, E.L., Stirewalt, D.L., Tait, J.F.& Tewari, M.2011.Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma.Proc Natl Acad Sci U S A, 108: 5003-5008.

Bala, S., Petrasek, J., Mundkur, S., Catalano, D., Levin, I., Ward, J., Alao, H., Kodys, K.& Szabo, G.2012.Circulating microRNAs in exosomes indicate hepatocyte injury and inflammation in alcoholic, drug-induced, and inflammatory liver diseases.Hepatology, 56, 1946-57.

Cortez, M.A., Bueso-Ramos, C., Ferdin, J., Lopez-Berestein, G., Sood, A.K.& Calin, G.A.2011.MicroRNAs in body fluids--the mix of hormones and biomarkers.Nat Rev Clin Oncol, 8, 467-77.

Devaux, Y.et al.Use of Circulating MicroRNAs to Diagnose Acute Myocardial Infarction.Clin Chem 58, 559-567, doi:10.1373/Clinchem.2011.173823 (2012).

Gandhi, R.et al.Circulating MicroRNAs as biomarkers for disease staging in multiple sclerosis.Ann Neurol 73, 729-740, doi:10.1002/Ana.23880 (2013).

Hanke, M., Hoefig, K., Merz, H., Feller, A.C., Kausch, I., Jocham, D., Warnecke, J.M.& Sczakiel, G.2010.A robust methodology to study urine microRNA as tumor marker: microRNA-126 and microRNA-182 are related to urinary bladder cancer.Urol Oncol, 28, 655-61.

Kohler, C., Barekati, Z., Radpour, R.& Zhong, X.Y.2011.Cell-free DNA in the circulation as a potential cancer biomarker.Anticancer Res, 31, 2623-8.

Laterza, O.F., Lim, L., Garrett-Engele, P.W., Vlasakova, K., Muniappa, N., Tanaka, W.K., Johnson, J.M., Sina, J.F., Fare, T.L., Sistare, F.D.& Glaab, W.E.2009.Plasma MicroRNAs as sensitive and specific biomarkers of tissue injury.Clin Chem, 55, 1977-83.

Lawrie, C.H., Gal, S., Dunlop, H.M., Pushkaran, B., Liggins, A.P., Pulford, K., Banham, A.H., Pezzella, F., Boultwood, J., Wainscoat, J.S., Hatton, C.S.& Harris, A.L.2008.Detection of elevated levels of tumour-associated microRNAs in serum of patients with diffuse large B-cell lymphoma.Br J Haematol, 141, 672-5.

Ma, Y., Zhang, P., Wang, F., Zhang, H., Yang, J., Peng, J., Liu, W.& Qin, H.2012.miR-150 as a potential biomarker associated with prognosis and therapeutic outcome in colorectal cancer.Gut, 61, 1447-53.

Mandel, P.& Métais, P.1948.Les acides nucléiques du plasma sanguin chez l’homme.C.R.Acad.Sci.Paris, 142, 241-243.

Mitchell, P.S., Parkin, R.K., Kroh, E.M., Fritz, B.R., Wyman, S.K., Pogosova-Agadjanyan, E.L., Peterson, A., Noteboom, J., O'Briant, K.C., Allen, A., Lin, D.W., Urban, N., Drescher, C.W., Knudsen, B.S., Stirewalt, D.L., Gentleman, R., Vessella, R.L., Nelson, P.S., Martin, D.B.& Tewari, M.2008.Circulating microRNAs as stable blood-based markers for cancer detection.Proc Natl Acad Sci U S A, 105, 10513-8.

Miyamoto, M., Yanai, M., Ookubo, S., Awasaki, N., Takami, K.& Imai, R.2008.Detection of cell-free, liver-specific mRNAs in peripheral blood from rats with hepatotoxicity: a potential toxicological biomarker for safety evaluation.Toxicol Sci, 106, 538-45.

Moltzahn, F.et al.Microfluidic-Based Multiplex qRT-PCR Identifies Diagnostic and Prognostic microRNA Signatures in the Sera of Prostate Cancer Patients.Cancer Res 71, 550-560, doi:10.1158/0008-5472.Can-10-1229 (2011).

Moussay, E., Wang, K., Cho, J., Van Moer, K., Pierson, S., Paggetti, J., Nazarov, P.V., Palissot, V., Hood, L., Berchem, G., & Galas, D.J.2011.microRNA as biomarkers and regulators in B chronic lymphocytic leukemia.PNAS, 108(16):6573-6578.

Okubo, S., Miyamoto, M., Takami, K., Kanki, M., Ono, A., Nakatsu, N., Yamada, H., Ohno, Y.& Urushidani, T.2013.Identification of novel liver-specific mRNAs in plasma for biomarkers of drug-induced liver injury and quantitative evaluation in rats treated with various hepatotoxic compounds.Toxicol Sci, 132, 21-31.

Park, N.J., Zhou, H., Elashoff, D., Henson, B.S., Kastratovic, D.A., Abemayor, E.& Wong, D.T.2009.Salivary microRNA: discovery, characterization, and clinical utility for oral cancer detection.Clin Cancer Res, 15, 5473-7.

Skog, J.et al.Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers.Nat Cell Biol 10, 1470-U1209, doi:10.1038/Ncb1800 (2008).

Su, Y.W., Chen, X., Jiang, Z.Z., Wang, T., Wang, C., Zhang, Y., Wen, J., Xue, M., Zhu, D., Zhang, Y., Su, Y.J., Xing, T.Y., Zhang, C.Y.& Zhang, L.Y.2012.A panel of serum microRNAs as specific biomarkers for diagnosis of compound- and herb-induced liver injury in rats.PLoS ONE, 7, e37395.

Sueoka, E., Sueoka, N., Iwanaga, K., Sato, A., Suga, K., Hayashi, S., Nagasawa, K.& Nakachi, K.2005.Detection of plasma hnRNP B1 mRNA, a new cancer biomarker, in lung cancer patients by quantitative real-time polymerase chain reaction.Lung Cancer, 48, 77-83.

Taylor, D.D.& Gercel-Taylor, C.2008.MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer.Gynecol Oncol, 110, 13-21.

Turchinovich, A., Weiz, L., Langheinz, A.& Burwinkel, B.2011.Characterization of extracellular circulating microRNA.Nucleic Acids Res, 39, 7223-33.

Vickers, K.C., Palmisano, B.T., Shoucri, B.M., Shamburek, R.D.& Remaley, A.T.2011.MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins.Nat Cell Biol, 13, 423-33.

Wagner, J., Riwanto, M., Besler, C., Knau, A., Fichtlscherer, S., Roxe, T., Zeiher, A.M., Landmesser, U.& Dimmeler, S.2013.Characterization of Levels and Cellular Transfer of Circulating Lipoprotein-Bound MicroRNAs.Arterioscler Thromb Vasc Biol.

Wang, G.K., Zhu, J.Q., Zhang, J.T., Li, Q., Li, Y., He, J., Qin, Y.W.& Jing, Q.2010.Circulating microRNA: a novel potential biomarker for early diagnosis of acute myocardial infarction in humans.Eur Heart J, 31, 659-66.

Wang, K., Li, H., Yuan, Y., Etheridge, A., Zhou, Y., Huang, D., Wilmes, P.& Galas, D.2012.The complex exogenous RNA spectra in human plasma: an interface with human gut biota? PLoS ONE, 7, e51009.

Wang, K.Yuan, Y., Cho, J-H., McClarty, S., Baxter, D.Galas, D.J.2012.Comparing the MicroRNA spectrum between serum and plasma.PLoS ONE, 7 (7), e41561

Wang, K., Zhang, S., Marzolf, B., Troisch, P., Brightman, A., Hu, Z., Hood, L.E.& Galas, D.J.2009.Circulating microRNAs, potential biomarkers for drug-induced liver injury.Proc Natl Acad Sci U S A, 106, 4402-7.

Weber, J.A., Baxter, D.H., Zhang, S., Huang, D.Y., Huang, K.H., Lee, M.J., Galas, D.J.& Wang, K.2010.The microRNA spectrum in 12 body fluids.Clin Chem, 56, 1733-41.

Witwer, K.W., McAlexander, M.A., Queen, S.E., and Adams R.J.2013.Real-time PCR and droplet digital PCR for plant miRNAs in mammalian blood provide little evidence for general uptake of dietary miRNAs.RNA Biology, 10:7, 1-7.

Wu, L., Zhou, H., Lin, H., Qi, J., Zhu, C., Gao, Z.& Wang, H.2012.Circulating microRNAs are elevated in plasma from severe preeclamptic pregnancies.Reproduction, 143, 389-97.

Zhang, X., Wang, C., Wang, L., Du, L., Wang, S., Zheng, G., Li, W., Zhuang, X., Zhang, X.& Dong, Z.2012b.Detection of circulating Bmi-1 mRNA in plasma and its potential diagnostic and prognostic value for uterine cervical cancer.Int J Cancer, 131, 165-72.

Zhao, H., Shen, J., Medico, L., Wang, D., Ambrosone, C.B.& Liu, S.2010.A pilot study of circulating miRNAs as potential biomarkers of early stage breast cancer.PLoS ONE, 5, e13735.

Zhao, Z., Zhao, Q., Warrick, J., Lockwood, C.M., Woodworth, A., Moley, K.H.& Gronowski, A.M.2012.Circulating microRNA miR-323-3p as a biomarker of ectopic pregnancy.Clin Chem, 58, 896-905.

Zhong, X.Y., Holzgreve, W.& Huang, D.J.2008.Isolation of cell-free RNA from maternal plasma.Methods Mol Biol, 444, 269-73.

Mechanisms & Models

Alexopoulou, L., Holt, A.C., Medzhitov, R., & Flavell, R.A. Recognition of double-stranded RNA and activation of NF-[kappa]B by Toll-like receptor 3. Nature(2001) 413: 732–738.

Bomberger, J.M., MacEachran, D.P., Coutermarsh, B.A., Ye, S., O’Toole, G.A., & Stanton, B.A.2009.Long-Distance Delivery of Bacterial Virulence Factors by Pseudomonas aeruginosa Outer Membrane Vesicles.PLoS Pathog, 5(4), e1000382.

Camussi, G., Deregibus, M.C., Bruno, S., Grange, C., Fonsato, V.& Tetta, C.2011.Exosome/microvesicle-mediated epigenetic reprogramming of cells.Am J Cancer Res, 1, 98-110.

Care, A., Catalucci, D., Felicetti, F., Bonci, D., Addario, A., Gallo, P., Bang, M.L., Segnalini, P., Gu, Y., Dalton, N.D., Elia, L., Latronico, M.V., Hoydal, M., Autore, C., Russo, M.A., Dorn, G.W., 2nd, Ellingsen, O., Ruiz-Lozano, P., Peterson, K.L., Croce, C.M., Peschle, C.& Condorelli, G.2007.MicroRNA-133 controls cardiac hypertrophy.Nat Med, 13, 613-8.

Chabelskaya, S., Gaillot, O., & Felden, B.2010.A Staphylococcus aureus Small RNA Is Required for Bacterial Virulence and Regulates the Expression of an Immune-Evasion Molecule.PLoS Pathog, 6(6), e1000927.

Chendrimada, T.P., Finn, K.J., Ji, X., Baillat, D., Gregory, R.I., Liebhaber, S.A., Pasquinelli, A.E.& Shiekhattar, R.2007.MicroRNA silencing through RISC recruitment of eIF6.Nature, 447, 823-8.

Choi, D.S., Kim, D.K., Kim, Y.K.& Gho, Y.S.Proteomics, transcriptomics and lipidomics of exosomes and ectosomes.Proteomics 13, 1554-1571, doi:10.1002/Pmic.201200329 (2013).

Gong, H., Vu, G.-P., Bai, Y., Chan, E., Wu, R., Yang, E., Liu, F., et al.2011.A Salmonella Small Non-Coding RNA Facilitates Bacterial Invasion and Intracellular Replication by Modulating the Expression of Virulence Factors.PLoS Pathog, 7(9), e1002120.

Hammell, C.M., & Hannon, G.J.2012.Inducing RNAi in C.elegans by Feeding with dsRNA-expressing E.coli.Cold Spring Harbor Protocols, 2012(12), pdb.prot072348.

Hansen, D.R., Van Alfen, N.K., Gillies, K., & Powell, W.A.1985.Naked dsRNA Associated with Hypovirulence of Endothia parasitica is Packaged in Fungal Vesicles.Journal of General Virology, 66(12), 2605–2614.

Kogure, T., Lin, W.L., Yan, I.K., Braconi, C.& Patel, T.2011.Intercellular nanovesicle-mediated microRNA transfer: a mechanism of environmental modulation of hepatocellular cancer cell growth.Hepatology, 54, 1237-48.

Higashijima, A., Miura, K., Mishima, H., Kinoshita, A., Jo, O., Abe, S., Hasegawa, Y., Miura, S., Yamasaki, K., Yoshida, A., Yoshiura, K.& Masuzaki, H.2013.Characterization of placenta-specific microRNAs in fetal growth restriction pregnancy.Prenat Diagn, 33, 214-22.

Lee, E.-Y., Choi, D.-Y., Kim, D.-K., Kim, J.-W., Park, J.O., Kim, S., Kim, S.-H., et al.2009.Gram-positive bacteria produce membrane vesicles: Proteomics-based characterization of Staphylococcus aureus-derived membrane vesicles.Proteomics, 9(24), 5425–5436.

Lee, S.T., Chu, K., Jung, K.H., Kim, J.H., Huh, J.Y., Yoon, H., Park, D.K., Lim, J.Y., Kim, J.M., Jeon, D., Ryu, H., Lee, S.K., Kim, M.& Roh, J.K.2012.miR-206 regulates brain-derived neurotrophic factor in Alzheimer disease model.Ann Neurol, 72, 269-77.

Liu, A.Y., Pascal, L.E., Vencio, R.Z.& Vencio, E.F.2010.Stromal-epithelial interactions in early neoplasia.Cancer biomarkers: section A of Disease markers, 9, 141-55.

Liu, H., Wang, X., Wang, H.-D., Wu, J., Ren, J., Meng, L., Wu, Q., et al.2012.Escherichia coli noncoding RNAs can affect gene expression and physiology of Caenorhabditis elegans.Nat Commun, 3, 1073.

Mittelbrunn, M., Gutierrez-Vazquez, C., Villarroya-Beltri, C., Gonzalez, S., Sanchez-Cabo, F., Gonzalez, M.A., Bernad, A.& Sanchez-Madrid, F.2011.Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.Nat Commun, 2, 282.

Parker, H., Chitcholtan, K., Hampton, M.B., & Keenan, J.I.2010.Uptake of Helicobacter pylori Outer Membrane Vesicles by Gastric Epithelial Cells.Infection and Immunity, 78(12), 5054–5061.

Ratajczak, J., Miekus, K., Kucia, M., Zhang, J., Reca, R., Dvorak, P.& Ratajczak, M.Z.2006.Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery.Leukemia, 20, 847-56.

Riley D.R., Sieber K.B., Robinson K.M., White J.R., Ganesan A., Nourbakhsh S., Dunning Hotopp J.C.2013.Bacteria-Human Somatic Cell Lateral Gene Transfer Is Enriched in Cancer Samples.PLoS Comput Biol, 9(6): e1003107.

Rodrigues, M.L., Franzen, A.J., Nimrichter, L., & Miranda, K.(n.d.).Vesicular mechanisms of traffic of fungal molecules to the extracellular space.Current Opinion in Microbiology, (0).doi:http://dx.doi.org/10.1016/j.mib.2013.04.002.

Schmidt, W.M., Spiel, A.O., Jilma, B., Wolzt, M.& Muller, M.2009.In vivo profile of the human leukocyte microRNA response to endotoxemia.Biochemical and biophysical research communications, 380, 437-41.

Valadi, H., Ekstrom, K., Bossios, A., Sjostrand, M., Lee, J.J.& Lotvall, J.O.2007.Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.Nat Cell Biol, 9, 654-9.

Vencio, E.F., Pascal, L.E., Page, L.S., Denyer, G., Wang, A.J., Ruohola-Baker, H., Zhang, S., Wang, K., Galas, D.J.& Liu, A.Y.2011.Embryonal carcinoma cell induction of miRNA and mRNA changes in co-cultured prostate stromal fibromuscular cells.Journal of cellular physiology, 226, 1479-88.

Wang, K., Zhang, S., Weber, J., Baxter, D., & Galas, D.J.2010a.Mammalian cells in culture actively export specific microRNAs.Nucleic Acids Research, 38(20):7248-7259.

Wang, K., Zhang, S., Weber, J., Baxter, D., & Galas, D.J.2010b.Export of microRNAs and microRNA-protective protein by mammalian cells.Nucleic Acids Res, 38, 7248-59.

Yang, M., Chen, J., Su, F., Yu, B., Lin, L., Liu, Y., Huang, J.D.& Song, E.2011.Microvesicles secreted by macrophages shuttle invasion-potentiating microRNAs into breast cancer cells.Molecular cancer, 10, 117.

Zhang, L., Hou, D., Chen, X., Li, D., Zhu, L., Zhang, Y., Li, J., Bian, Z., Liang, X., Cai, X., Yin, Y., Wang, C., Zhang, T., Zhu, D., Zhang, D., Xu, J., Chen, Q., Ba, Y., Liu, J., Wang, Q., Chen, J., Wang, J., Wang, M., Zhang, Q., Zhang, J., Zen, K.& Zhang, C.Y.2012a.Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA.Cell Res, 22, 107-26.

Methods/Protocols

Argyropoulos, C., Wang, K., McClarty, S., Huang, D., Bernardo, J., Ellis, D., Orchard, T., Galas, D., Johnson, J. 2013. Urinary MicroRNA Profiling in the Nephropathy of Type 1 Diabetes. PLoS ONE 8:e54662. doi: 10.1371/journal.pone.0054662.

Lee, H.-J., & Hong, S.-H. 2012. Analysis of microRNA-size, small RNAs in Streptococcus mutans by deep sequencing. FEMS Microbiology Letters, 326(2), 131–136.

Lee, L.W., Zhang, S., Etheridge, A., Ma, L., Martin, D., Galas, D. & Wang, K. 2010. Complexity of the microRNA repertoire revealed by next-generation sequencing. RNA, 16, 2170-80.

Kang, S.M., Choi, J.W., Lee, Y., Hong, S.H., & Lee, H.-J. 2013. Identification of microRNA-Size, Small RNAs in Escherichia coli. Current Microbiology, 1–5. doi:10.1007/s00284-013-0411-9.

Kogure, T., & Patel, T.  2013.   Isolation of extracellular nanovesicle microRNA from liver cancer cells in culture.  Methods Mol Biol. 2013;1024:11-8. doi: 10.1007/978-1-62703-453-1_2.

Nolte-'T hoen, E.N.M., Buermans, H.P.J., Wasdorp, M., Stoorvogel, W., Wauben, M.H.M., & 'T hoen, P.A.C., Deep sequencing of RNA from immune cell-dervived vesicles uncovers the selective incorporation of small non-coding RNA biotypes with potential regulatory functions,  Nucl Acids Res. (2012) 40: 9272-9285.

Semenov, D.V., Baryakin, D.N., Brenner, E.V., Kurilshikov, A.M., Vasiliev, G.V., Bryzgalov, L.A., Chikova, E.D., Filippova, J.A., Kuligina, E.V. & Richter, V.A. 2012. Unbiased approach to profile the variety of small non-coding RNA of human blood plasma with massively parallel sequencing technology. Expert Opin Biol Ther, 12 Suppl 1, S43-51.

Sipova, H., Zhang, S., Dudley, A.M., Galas, D.J., Wang, K. & Homola, J. 2010. Surface Plasmon Resonance Biosensor for Rapid Label-free Detection of microRNA at Sub-femtomolar Levels. Analytical Chemistry, 82(24):10110-10115.

Turchinovich A., Weiz, L., Langheinz, A., & Burwinkel B. 2011. Characterization of extracellular circulating microRNA.  Nucleic Acids Res. 39(16):7223-33. doi: 10.1093/nar/gkr254. Epub 2011 May 24.

Wang, Q.L. et al. Delivery of therapeutic agents by nanoparticles made of grapefruit-derived lipids. Nat Commun 4, 1867, doi:10.1038/Ncomms2886 (2013).

Witwer, K.W. et al. Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. J Extracell Ves 2, 20360, doi:10.3402/jev.v2i0.20360 (2013).