Protocols

Protocols

   

The consortium is working internally to compare Standard Operating Procedures (SOPs), i.e. protocols used by different labs for extracellular vesicle preparation and RNA analysis. Vetted results are here.

   

Protocols Decision Tree

This decision tree allows a beginner to understand the best approaches available in the field to study extracellular vesicles and extracellular RNA. See this blog post for an in-depth discussion of how to select protocols from this page.

 

Collection of Biofluids

 

Isolation and Enrichment of Extracellular Vesicles

 

RNA Isolation

 

Validation and Quantification

 

RNA Sequencing

 

Proteomics on Extracellular Vesicles