The ERCC Resource Catalog was initiated by Mike McManus of UCSF and Al Charest of Harvard of the U19 Working Group during the first stage of the ERCC. The U19 WG consisted of 5 diverse research teams in San Francisco, Boston, Nashville, and New York whose mandate was to understand the biological function and mechanism of exRNAs. (See the project description here.)

A part of that mandate was to make available to the wider research community any useful tools and reagents developed during the course of their research. As ERCC Stage 1 ends, we are working to transition the still useful plasmids, cell lines, and mouse strains into public repositories.

The process is ongoing –– we list here only those materials that we have vetted and know to be easily available and useful for future research. The original full catalog is still available on the Resources/Reagents page for those looking for something specific, and the academic literature is always the best source of information about research materials. Our Resources and Technology publications and Protocols pages are good starting points.

For sharing of archived biological samples, go to the Virtual Biorepository, and to explore our dataset comparing the performance of 10 RNA isolation kits in 5 biofluids, go to the miRDaR app.

If your lab has resources that you think would benefit the exRNA and EV research communities, please contact us at info@exRNA.org so that we can work together to make them widely available.

Contact PI Co-PI Availability Plasmid Description Comments Point of Contact email Publication
 
Blelloch Huang pcDNA3.1-GFP(1-10) GFP1-10 fragment for the fluorescent labeling of GFP11 fusion proteins Complementation between GFP1-10 and GFP11-fusion proteins recreates GFP, allowing the imaging of GFP11 fusion proteins bo.huang@ucsf.edu PMID 26988139
Blelloch Huang
pEGFP-GFP11-Clathrin light chain
GFP11 labeled clathrin As a positive control for GFP1-10 and GFP11 complementation bo.huang@ucsf.edu PMID 26988139
Blelloch Huang
pACUH-GFP11x7-mCherry-ß-tubulin
7x tandem GFP11 labeled tubulin 7x GFP11 tandem labeling amplifies the fluorescence signal from individual fusion proteins by a factor of 7 bo.huang@ucsf.edu PMID 26988139
Blelloch Huang pcDNA3.1-sfCherry(1-10) sfCherry1-10 fragment for the fluorescent labeling of sfCherry11 fusion proteins Complementation between sfCherry1-10 and sfCherry11-fusion proteins recreates sfCherry (having the same fluorescence properties as mCherry), allowing two-color imaging togethe with GFP bo.huang@ucsf.edu PMID 26988139
Blelloch Huang
pEGFP-sfCherry11-ß-actin
sfCherry11 labeled actin As a positive control for sfCherry1-10 and sfCherry11 complementation bo.huang@ucsf.edu PMID 26988139
Blelloch Huang
pEGFP-sfCherry11x4-ß-actin
7x tandem sfCherry11 labeled actin 4x sfCherry11 tandem labeling amplifies the fluorescence signal from individual fusion proteins by a factor of 4 bo.huang@ucsf.edu PMID 26988139
Blelloch Huang pHR-SFFV-GFP1-10 Lentiviral vector for the generation of GFP1-10 stable cell lines GFP1-10 stable cell lines enable easy fluorescent tagging of endogenous genes via GFP11 knock-in bo.huang@ucsf.edu PMID 27274053
Blelloch Huang Share via collaboration pHR-SFFV-sfCherry+1-10 Lentiviral vector for the generation of sfCherry+1-10 (improved version) stable cell lines sfCherry+1-10 stable cell lines enable easy fluorescent tagging of endogenous genes via sfCherry+11 knock-in; sfCherry+ is an improved version of sfCherry bo.huang@ucsf.edu Unpublished
Blelloch Huang Share via collaboration
pcDNA3.1-PAsfCherry+1-10
Photoactivatable version of sfCherry+ for protein tracking ans super-resolution microscopy PAsfCherry+1-10 is a photoactivatible mutant of sfCherry+ bo.huang@ucsf.edu Unpublished