The ERCC Resource Catalog was initiated by Mike McManus of UCSF and Al Charest of Harvard of the U19 Working Group during the first stage of the ERCC. The U19 WG consisted of 5 diverse research teams in San Francisco, Boston, Nashville, and New York whose mandate was to understand the biological function and mechanism of exRNAs. (See the project description here.)
A part of that mandate was to make available to the wider research community any useful tools and reagents developed during the course of their research. As ERCC Stage 1 ends, we are working to transition the still useful plasmids, cell lines, and mouse strains into public repositories.
The process is ongoing –– we list here only those materials that we have vetted and know to be easily available and useful for future research. The original full catalog is still available on the Resources/Reagents page for those looking for something specific, and the academic literature is always the best source of information about research materials. Our Resources and Technology publications and Protocols pages are good starting points.
|Contact PI||Co-PI||Availability||Plasmid||Description||Comments||Point of Contact email||Publication|
|Blelloch||Huang||pcDNA3.1-GFP(1-10)||GFP1-10 fragment for the fluorescent labeling of GFP11 fusion proteins||Complementation between GFP1-10 and GFP11-fusion proteins recreates GFP, allowing the imaging of GFP11 fusion email@example.com||PMID 26988139|
pEGFP-GFP11-Clathrin light chain
|GFP11 labeled clathrin||As a positive control for GFP1-10 and GFP11 firstname.lastname@example.org||PMID 26988139|
|7x tandem GFP11 labeled tubulin||7x GFP11 tandem labeling amplifies the fluorescence signal from individual fusion proteins by a factor of email@example.com||PMID 26988139|
|Blelloch||Huang||pcDNA3.1-sfCherry(1-10)||sfCherry1-10 fragment for the fluorescent labeling of sfCherry11 fusion proteins||Complementation between sfCherry1-10 and sfCherry11-fusion proteins recreates sfCherry (having the same fluorescence properties as mCherry), allowing two-color imaging togethe with GFPfirstname.lastname@example.org||PMID 26988139|
|sfCherry11 labeled actin||As a positive control for sfCherry1-10 and sfCherry11 email@example.com||PMID 26988139|
|7x tandem sfCherry11 labeled actin||4x sfCherry11 tandem labeling amplifies the fluorescence signal from individual fusion proteins by a factor of firstname.lastname@example.org||PMID 26988139|
|Blelloch||Huang||pHR-SFFV-GFP1-10||Lentiviral vector for the generation of GFP1-10 stable cell lines||GFP1-10 stable cell lines enable easy fluorescent tagging of endogenous genes via GFP11 email@example.com||PMID 27274053|
|Blelloch||Huang||Share via collaboration||pHR-SFFV-sfCherry+1-10||Lentiviral vector for the generation of sfCherry+1-10 (improved version) stable cell lines||sfCherry+1-10 stable cell lines enable easy fluorescent tagging of endogenous genes via sfCherry+11 knock-in; sfCherry+ is an improved version of sfCherryfirstname.lastname@example.org||Unpublished|
|Blelloch||Huang||Share via collaboration|
|Photoactivatable version of sfCherry+ for protein tracking ans super-resolution microscopy||PAsfCherry+1-10 is a photoactivatible mutant of sfCherryemail@example.com||Unpublished|