EV Antibody Database

Western Blot Antibody

Target protein:

CD234 (DARC)

Primary antibody:

Abcam ab137044 (Rabbit)

No notes

Source material:

Not specified

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Secondary antibody:

Cell Signaling 7074S (Goat)

No notes

Lysis Conditions:

RIPA buffer
at
22°C
for
1 min

Gel Conditions

Minigel, 10 % acrylamide

 

Gel type:

BioRad 4568035

Run conditions:

200 V / 0 Amps for 0.5 hr

Protein loading:

20 𝜇g

Reducing conditions:

β-mercaptoethanol

No notes

Transfer Conditions

Membrane type:

PVDF

Transfer method:

Wet

Transfer buffer:

Tris-glycine with 10% methanol

Transfer voltage:

100 V

Transfer current:

0 Amps

Transfer temperature:

4°C

Transfer duration:

1 hr

Blocking

Blocking buffer:

Superblock

Blocking temperature:

22°C

Duration:

1 hr

Primary Incubation

Antibody 1 dilution

1:10

Incubation temperature

22°C

Incubation duration

1 hr

Number of washes

3

Wash buffer

TBST

Wash duration

5 min

Secondary Incubation

Antibody 2 dilution

1:1000

Incubation temperature

22°C

Incubation duration

1 hr

Number of washes

3

Wash buffer

TBST

Wash duration

5 min

Detection

Detection method

Enhanced chemiluminescence (ECL)

Expected band size

37 kDa

Result:

Weak single band

Background:

High background

Related Publications

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No comments

Record 3190 author: Amber Morey

Author Information

Email:moreyamber@gmail.com
Lab Head:
Institution:
Website:https://obgyn.ucsd.edu/research/labs/laurent/
Last edit: Thu Feb 23, 2023