Primary antibody: Novus Biologicals NBP2-42225 (Mouse)
Vendor Link Antibodypedia Link
Source material: Biofluid: Human Plasma
EV was extracted from 1 ml of plasma using a Size-Exclusion Chromatography column (qEV Original, 35nm, Izon Science) according to manufacturer's instructions.
Secondary antibody: Dako p0447 (Mouse)
Lysis Conditions: SDS
at
95°C
for
7 min
Gel Conditions Run conditions: 120 V / 0 Amps for 60 min
Reducing conditions: β-mercaptoethanol
Transfer Conditions Membrane type: PVDF
Transfer method: Semi-dry
Transfer buffer: Tris-glycine with 20% methanol
Transfer voltage: 25 V
Transfer current: 2.5 Amps
Transfer temperature: 22°C
Transfer duration: 0.16 hr
Blocking Blocking buffer: 0% milk, 0% BSA, No serum
Blocking temperature: 22°C
Duration: 1 hr
Primary Incubation Antibody 1 dilution 1:1000
Incubation temperature 4°C
Incubation duration 16 hr
Number of washes 4
Wash buffer TBST
Wash duration 10 min
Secondary Incubation Antibody 2 dilution 1:5,000
Incubation temperature 22°C
Incubation duration 1 hr
Number of washes 4
Wash buffer TBST
Wash duration 10 min
Detection Record 9712 author: Michail Spanos
Last edit: Tue Mar 19, 2024
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